Analysis of Tyr aminoacyl synthetase.

Analysis performed on 2024-09-30 10:31:03

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 44519 798 43721 11122
Archaea 3360 91 3269 1434
Vertebrata 1161 312 849 424
Mammals 491 68 423 124

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
528 29 418

Domains

Domains
Current Study 348, 529
Guo et al (2010) 360, 524

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 346 182 528 1.901099
Number of Variants 284 163 447 1.742331
Number of Pathogenic Variants 23 6 29 3.833333
Number of Benign/Unknown Variants 261 157 418 1.662420
Fisher exact test 0.074675

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 363 165 528 2.200000
Number of Variants 303 144 447 2.104167
Number of Pathogenic Variants 24 5 29 4.800000
Number of Benign/Unknown Variants 279 139 418 2.007194
Fisher exact test 0.098910

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 346 182 0.92 (0.01) 0.86 (0.01) 9.575e-01 9.123e-01 40896.00 8.10e-09
Guo et al (2010) 363 165 0.91 (0.01) 0.87 (0.01) 9.571e-01 9.165e-01 37190.00 4.15e-06

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains